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The development of a test system for determining antibodies to Bacteroides melaninogenicus using solid-phase immunoenzyme analysis

Tsarev, V.N.; Ushakov, R.V.; Vaneeva, N.P.; Iastrebova, N.E.; Ushakova, T.V.

Klinicheskaia Laboratornaia Diagnostika 5: 11-15

1993

ISSN/ISBN: 0869-2084
PMID: 7994533
Document Number: 421913
A test system for enzyme immunoassay of antibodies to Bacteroides melaninogenicus has been developed. The authors describe a method for the preparation of the protein and polysaccharide antigens with the use of ultrasonic, lysozyme, and beta-naphthol treatment. A total of 105 blood serum samples, 46 samples of gingival blood and gingival fluid from patients with maxillofacial phlegmons and periodontitis were examined, making use of the 1 +/- 3 omega index (from the values in healthy donors). The results evidence statistically significant difference in antibody levels of patients from whom B. melaninogenicus were isolated and of those with the negative results of bacteriologic analysis. The titers of antibodies to B. melaninogenicus in the blood and gingival fluid of patients with periodontitis were in good correlation.

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Gervazieva, V.B.; Ovsiannikova, I.G.; Voronkin, N.I.; Sorochinskaia, N.N.; Raĭkis, B.N. 1987: Use of solid-phase immunoenzyme analysis for determining allergen-specific IgE antibodies Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 9: 33-35
Meshandin, A.G.; Dargeeva, T.A.; Vaneeva, L.I. 1993: The use of the physicochemical laws governing the sorption properties of antigens (antibodies) in relation to the plastic solid phase for enhancing the sensitivity of immunoenzyme analysis test systems in the diagnosis of infections Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 92-95
Litvinovich, N.E.; Nosik, D.N.; Novokhatskiĭ, A.S. 1986: Optimization of conditions for preparation of the solid phase of infected cells in the immunoenzyme analysis of monoclonal antibodies Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 5: 39-44
Rott, G.M.; Lapshina, G.M.; Rozhinskaia, I.V.; Letova, O.A.; Blinova, T.V. 1988: Development and comparisons of information value of different methods of myoglobin determination: solid phase immunoenzyme assay and hemagglutination test Voprosy Meditsinskoi Khimii 34(5): 124-129
Buglanov, A.A.; Kazakbaeva, K.M.; Salikhov, T.A. 1988: The solid-phase immunoenzyme method of determining the ferritin concentration of human blood serum Gematologiia i Transfuziologiia 33(5): 53-54
Umnova, N.S.; Shakhanina, K.L.; Meshcheriakova, I.S.; Pavlova, I.P. 1984: Determination of tularemia antibodies by an immunoenzyme method on a solid-phase carrier (ELISA) Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 79-83
Priimak, A.A.; Vladimirskiĭ, M.A.; Ermolin, G.A.; Dolzhanskiĭ, V.M. 1986: Use of an immunoenzyme solid-phase method of detecting antibodies to mycobacteria for the serologic diagnosis of tuberculosis Laboratornoe Delo 9: 558-562
Iuronen, E.I.; Viĭksmaa, M.G.; Mikel'saar, A.V. 1988: The use of monoclonal antibodies for the quantitative determination of myoglobin by the solid phase immunoenzyme "sandwich" assay Voprosy Meditsinskoi Khimii 34(4): 132-136
Arenkov, P.I.; Zhmareva, E.N.; Berezin, V.A. 1989: A method of solid phase immunoenzyme analysis using antigen molecules immobilized on membranes Ukrainskii Biokhimicheskii Zhurnal 61(6): 89-92
Chernokhvostova, E.V.; Kotova, T.S.; Atovmian, O.I.; Arsen'eva, E.L.; Bogacheva, G.T.; Rokhlin, O.V. 1989: Immunoenzyme test system with monoclonal antibodies to human IgG4 in the determination of allergen-specific antibodies in pollinosis Biulleten' Eksperimental'noi Biologii i Meditsiny 108(11): 574-577
Ivanov, K.S.; Rybalko, V.V.; Finogeev, I.P.; Luk'ianov, V.V. 1993: Solid-phase immunoenzyme analysis in the differential diagnosis of pulmonary tuberculosis and other infectious diseases with respiratory organ involvement Voenno-Meditsinskii Zhurnal 1993(5): 41
Kanev, A.N.; Vorob'eva, M.S.; Zagrebel'nyĭ, S.N.; Shirokikh, E.I.; Gorbatov, A.F.; Usova, S.V.; Lomanova, G.A. 1992: The principles for standardizing serum panels intended for the quality assessment of diagnostic test systems (exemplified by an immunoenzyme test system for detecting HIV antibodies) Voprosy Virusologii 37(2): 122-124
Kuz'mina, T.I.; Cherkasskiĭ, B.L. 1991: An immunoenzyme test system for the detection of antibodies to Campylobacter Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 10: 47-50
Serdiuk, O.A.; Tsytsikov, E.N.; Vasilov, R.G. 1989: Monoclonal antibody-based immunoenzyme test system for determining the total immunoglobulin e level of children and adolescents Biulleten' Eksperimental'noi Biologii i Meditsiny 107(6): 722-724
Kibardin, V.M.; Ebralidze, L.K.; Zvonarev, A.I. 1989: A simple and rapid method of determining the titer of herpes simplex virus antibodies in a study by immunoenzyme analysis of a single serum dilution Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 1: 61-63
Iagovkina, V.V.; Moskalenko, E.P.; Levi, M.I. 1988: Development of an immunoenzyme test system for the diagnosis of pertussis using penicillinase conjugates Laboratornoe Delo 6: 62-65
Borisevich, I.V.; Mikhaĭlov, V.V.; Potryvaeva, N.V.; Malinkin, I.N.; Kirillov, A.P.; Krasnianskiĭ, V.P.; Markov, V.I.; Makhlaĭ, A.A.; Lebedinskaia, E.V. 1996: Development of the immunoenzyme test-system for detection of Ebola virus antigen Voprosy Virusologii 41(5): 232-234
Rezapkin, G.V.; Tkachenko, E.A.; Ivanov, A.P.; Bashkirtsev, V.N.; Dzagurova, T.K. 1981: Determination of arenavirus antigens and antibodies by solid-phase radioimmunological analysis Voprosy Virusologii 4: 459-462
Vinskaia, A.I.; Grigorashkin, I.I. 1992: The quantitative detection of orthopoxvirus antibodies in studying sera in a single dilution in an immunoenzyme test system with a conjugate of protein a and peroxidase Voprosy Virusologii 37(2): 124-127
Trushinskaia, G.N.; Simonov, V.I.; Makarova, N.E.; Nosik, D.N.; Kushch, A.A.; Gibadulin, R.A. 1992: The immunoenzyme test system for detection of HIV-1 antigens based on using immune polyclonal anti-HIV serum and monoclonal antibodies against gene GAG HIV-1 proteins Vestnik Rossiiskoi Akademii Meditsinskikh Nauk 1992(9-10): 43-47