Top Abstract Purchase Related
Home
>
Node
>
Subnode

The use of monoclonal antibodies for the quantitative determination of myoglobin by the solid phase immunoenzyme "sandwich" assay

Iuronen, E.I.; Viĭksmaa, M.G.; Mikel'saar, A.V.

Voprosy Meditsinskoi Khimii 34(4): 132-136

1988

ISSN/ISBN: 0042-8809
PMID: 3057738
Document Number: 317661
A procedure of solid phase single step immunoenzyme assay of the "sandwich" type involving monoclonal antibodies was developed to estimate myoglobin in blood serum of patients, 30 stable hybridomic clones. producing antibodies to not less than six various epitopes of myoglobin, were obtained. Content of myoglobin in blood serum was studied using antibodies of the IgG type to two various epitopes of myoglobin as well as of their conjugates with horseradish peroxidase. The procedure developed enabled to estimate myoglobin in blood serum at concentrations 2-1,600 ng/ml and to analyze 120 preparations within 3 hrs.

Document emailed within 1 workday
Secure & encrypted payments

Rott, G.M.; Lapshina, G.M.; Rozhinskaia, I.V.; Letova, O.A.; Blinova, T.V. 1988: Development and comparisons of information value of different methods of myoglobin determination: solid phase immunoenzyme assay and hemagglutination test Voprosy Meditsinskoi Khimii 34(5): 124-129
Litvinovich, N.E.; Nosik, D.N.; Novokhatskiĭ, A.S. 1986: Optimization of conditions for preparation of the solid phase of infected cells in the immunoenzyme analysis of monoclonal antibodies Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 5: 39-44
Umnova, N.S.; Shakhanina, K.L.; Meshcheriakova, I.S.; Pavlova, I.P. 1984: Determination of tularemia antibodies by an immunoenzyme method on a solid-phase carrier (ELISA) Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 79-83
Habermann, E.; Bernáth, S. 1975: Two solid-phase radioimmunoassays for the quantitative determination of Clostridium botulinum type-A antibodies Acta Veterinaria Academiae Scientiarum Hungaricae 25(4): 377-382
Di Giacomo, A.; di Matteo, L.; Tranchese, D.; Dericoloso, A.; De Vito, R.; Zinzi, V. 1984: Use of solid-phase monoclonal antibodies in the determination of peripheral lymphocyte classes in cancer patients Archivio Monaldi Per la Tisiologia E le Malattie Dell'apparato Respiratorio 39(4): 313-318
Mezzano, S.; López, M.I. 1984: Detection of circulating immune complexes by immunoenzyme assay with solid phase Clq Revista Medica de Chile 112(6): 547-552
Pervushina, E.A.; Kopylov, A.V.; Rogova, S.S.; Pervushin, I.V. 2010: Possibility of detecting HBsAG in bloodstains on evidential objects by solid-phase immunoenzyme assay Sudebno-Meditsinskaia Ekspertiza 53(1): 22-25
Gervazieva, V.B.; Ovsiannikova, I.G.; Voronkin, N.I.; Sorochinskaia, N.N.; Raĭkis, B.N. 1987: Use of solid-phase immunoenzyme analysis for determining allergen-specific IgE antibodies Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 9: 33-35
Eremenko, S.N.; Sukhov, I.E.; Miroshnikov, A.I. 1993: Determination of cardenolides in Digitalis lanata by a solid-phase immunoenzyme method Bioorganicheskaia Khimiia 19(10): 955-960
Chernokhvostova, E.V.; Kotova, T.S.; Atovmian, O.I.; Arsen'eva, E.L.; Bogacheva, G.T.; Rokhlin, O.V. 1989: Immunoenzyme test system with monoclonal antibodies to human IgG4 in the determination of allergen-specific antibodies in pollinosis Biulleten' Eksperimental'noi Biologii i Meditsiny 108(11): 574-577
Priimak, A.A.; Vladimirskiĭ, M.A.; Ermolin, G.A.; Dolzhanskiĭ, V.M. 1986: Use of an immunoenzyme solid-phase method of detecting antibodies to mycobacteria for the serologic diagnosis of tuberculosis Laboratornoe Delo 9: 558-562
Mosmann, T.R.; Schumacher, J.H.; Fiorentino, D.F.; Leverah, J.; Moore, K.W.; Bond, M.W. 1990: Isolation of monoclonal antibodies specific for IL-4, IL-5, IL-6, and a new Th2-specific cytokine (IL-10), cytokine synthesis inhibitory factor, by using a solid phase radioimmunoadsorbent assay Journal of Immunology 145(9): 2938-2945
Tsarev, V.N.; Ushakov, R.V.; Vaneeva, N.P.; Iastrebova, N.E.; Ushakova, T.V. 1993: The development of a test system for determining antibodies to Bacteroides melaninogenicus using solid-phase immunoenzyme analysis Klinicheskaia Laboratornaia Diagnostika 5: 11-15
Belozerov, A.P. 1989: A method of isolating C1q from human serum and its use in the solid-phase immunoenzyme determination of immune complexes Laboratornoe Delo 3: 24-26
Ruelland, A.; Gobron, S.; Clerc, C.; Legras, B.; Heusghem, C.; Cloarec, L. 1986: Use of monoclonal antibodies for the immunoenzyme determination of creatine kinase-isoenzyme MB Pathologie-Biologie 34(2): 145-147
Meshandin, A.G.; Dargeeva, T.A.; Vaneeva, L.I. 1993: The use of the physicochemical laws governing the sorption properties of antigens (antibodies) in relation to the plastic solid phase for enhancing the sensitivity of immunoenzyme analysis test systems in the diagnosis of infections Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 92-95
Ivanov, A.P.; Rezapkin, G.V.; Dzagurova, T.K.; Tkachenko, E.A. 1984: Indirect solid-phase immunosorbent assay for detection of arenavirus antigens and antibodies Acta Virologica 28(3): 240-245
Rezapkin, G.V.; Tkachenko, E.A.; Ivanov, A.P.; Bashkirtsev, V.N.; Dzagurova, T.K. 1981: Determination of arenavirus antigens and antibodies by solid-phase radioimmunological analysis Voprosy Virusologii 4: 459-462
Xu, Z.Y. 1984: A solid-phase double-sandwich ELISA for detecting anti-HBc IgM Zhonghua Liu Xing Bing Xue Za Zhi 5(3): 176-179
Humbert, L.; Dehon, B.; Decaestecker, B.; Mathieu, D.; Houdret, N.; Lhermitte, M. 1999: Identification and quantitative determination of chlorinated++ solvents after solid phase microextraction. Applications in hospital toxicology Acta Clinica Belgica 53(Suppl 1): 89-93