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Determination of cardenolides in Digitalis lanata by a solid-phase immunoenzyme method

Eremenko, S.N.; Sukhov, I.E.; Miroshnikov, A.I.

Bioorganicheskaia Khimiia 19(10): 955-960

1993

ISSN/ISBN: 0132-3423
PMID: 8274170
Document Number: 410463
A competitive solid-phase EIA technique using high-affinity digoxin-specific monoclonal antibodies has been developed to determine cardenolides in Digitalis lanata. The test-system can detect digoxin and closely related compounds in concentration of 0.1 nmol/ml. The content of cardenolides, tested by ELISA, correlated well with the total content of digoxin, deslanoside C and lanatoside C as analysed by HPLC. The test-system is useful in estimating the productivity of large series and samples of tissue clones of Digitalis lanata.

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