A method of solid phase immunoenzyme analysis using antigen molecules immobilized on membranes
Arenkov, P.I.; Zhmareva, E.N.; Berezin, V.A.
Ukrainskii Biokhimicheskii Zhurnal 61(6): 89-92
1989
ISSN/ISBN: 0201-8470 PMID: 2698529 Document Number: 342471
The enzyme-linked immunoassay modification has been worked out. The method combines advantages of membrane technology of antigen immobilization which is used in the enzyme immunosensory technique and of conventional enzyme-linked immunosorbent assay. The nitrocellulose and polypropylene membranes are used as a solid-phase. The purified rabbit immunoglobulin G is immobilized on the surface of membranes as the first layer. The competitive immunoassay is employed. The immunoglobulin G concentration range is 1-1000 ng/ml. The membranes with the immobilized antigen can be repeatedly used after incubation in 0.1 M glycine buffer, pH 2.5. The dry membrane with the immobilized antigen can be used after keeping for 6 months in refrigerator at 4 degrees C without changing the concentration range measured.