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Determination of tularemia antibodies by an immunoenzyme method on a solid-phase carrier (ELISA)

Umnova, N.S.; Shakhanina, K.L.; Meshcheriakova, I.S.; Pavlova, I.P.

Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 79-83

1984

ISSN/ISBN: 0372-9311
PMID: 6377770
Document Number: 230055
ELISA "sandwich" techniques have been developed and the optimum assay conditions for detecting specific antibodies in human serum samples have been determined. The possibility of using these techniques for the determination of the level of antibodies to tularemia antigens in the sera of persons immunized with live tularemia vaccine has been shown. Statistically significant differences in the level of antibodies to tularemia antigen in the sera of immunized and nonimmunized persons have been established. The comparative study of five serological methods - ELISA, the agglutination test, the passive hemagglutination test, the immunofluorescence test and the defined antigen substrate sera ( DASS ) techniques - has revealed the advantage of ELISA, whose sensitivity has proved to be considerably higher than that of all other methods used in our work.

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Iuronen, E.I.; Viĭksmaa, M.G.; Mikel'saar, A.V. 1988: The use of monoclonal antibodies for the quantitative determination of myoglobin by the solid phase immunoenzyme "sandwich" assay Voprosy Meditsinskoi Khimii 34(4): 132-136
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Priimak, A.A.; Vladimirskiĭ, M.A.; Ermolin, G.A.; Dolzhanskiĭ, V.M. 1986: Use of an immunoenzyme solid-phase method of detecting antibodies to mycobacteria for the serologic diagnosis of tuberculosis Laboratornoe Delo 9: 558-562
Belozerov, A.P. 1989: A method of isolating C1q from human serum and its use in the solid-phase immunoenzyme determination of immune complexes Laboratornoe Delo 3: 24-26
Gervazieva, V.B.; Ovsiannikova, I.G.; Voronkin, N.I.; Sorochinskaia, N.N.; Raĭkis, B.N. 1987: Use of solid-phase immunoenzyme analysis for determining allergen-specific IgE antibodies Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 9: 33-35
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Litvinovich, N.E.; Nosik, D.N.; Novokhatskiĭ, A.S. 1986: Optimization of conditions for preparation of the solid phase of infected cells in the immunoenzyme analysis of monoclonal antibodies Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 5: 39-44
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Arenkov, P.I.; Zhmareva, E.N.; Berezin, V.A. 1989: A method of solid phase immunoenzyme analysis using antigen molecules immobilized on membranes Ukrainskii Biokhimicheskii Zhurnal 61(6): 89-92
Buglanov, A.A.; Kazakbaeva, K.M.; Salikhov, T.A. 1988: The solid-phase immunoenzyme method of determining the ferritin concentration of human blood serum Gematologiia i Transfuziologiia 33(5): 53-54
Rott, G.M.; Lapshina, G.M.; Rozhinskaia, I.V.; Letova, O.A.; Blinova, T.V. 1988: Development and comparisons of information value of different methods of myoglobin determination: solid phase immunoenzyme assay and hemagglutination test Voprosy Meditsinskoi Khimii 34(5): 124-129
Meshandin, A.G.; Dargeeva, T.A.; Vaneeva, L.I. 1993: The use of the physicochemical laws governing the sorption properties of antigens (antibodies) in relation to the plastic solid phase for enhancing the sensitivity of immunoenzyme analysis test systems in the diagnosis of infections Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 92-95
Gombosová, A.; Valent, M. 1988: Use of the ELISA method in the determination of antibodies to Trichomonas vaginalis Bratislavske Lekarske Listy 89(9): 689-693
Rezapkin, G.V.; Tkachenko, E.A.; Ivanov, A.P.; Bashkirtsev, V.N.; Dzagurova, T.K. 1981: Determination of arenavirus antigens and antibodies by solid-phase radioimmunological analysis Voprosy Virusologii 4: 459-462
Salamova, R.E.; Gorina, L.G.; Volkova, I.I. 1985: Determination of Mycoplasma pneumoniae antigens and antibodies by the immunoenzyme method in patients with respiratory tract diseases Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 7: 48-51
Koval', A.A.; Mazurov, A.V.; Vinogradov, D.V.; Rudin, A.V.; Repin, V.S. 1989: Determination of antithrombocyte antibodies in the blood serum of patients with idiopathic thrombocytopenic purpura by an immunoenzyme method Biulleten' Eksperimental'noi Biologii i Meditsiny 107(3): 327-329
Habermann, E.; Bernáth, S. 1975: Two solid-phase radioimmunoassays for the quantitative determination of Clostridium botulinum type-A antibodies Acta Veterinaria Academiae Scientiarum Hungaricae 25(4): 377-382
Di Giacomo, A.; di Matteo, L.; Tranchese, D.; Dericoloso, A.; De Vito, R.; Zinzi, V. 1984: Use of solid-phase monoclonal antibodies in the determination of peripheral lymphocyte classes in cancer patients Archivio Monaldi Per la Tisiologia E le Malattie Dell'apparato Respiratorio 39(4): 313-318
Chagiashvili, T.N.; Zotikov, E.A. 1994: Solid-phase method in determination of blood groups and rhesus factor Klinicheskaia Laboratornaia Diagnostika 4: 33
Jansson, C. 2000: Multiresidue method for the gas chromatographic determination of pesticides in honey after solid-phase extraction cleanup Journal - Association of Official Analytical Chemists 83(3): 714-719