Monoclonal antibody-based immunoenzyme test system for determining the total immunoglobulin e level of children and adolescents
Serdiuk, O.A.; Tsytsikov, E.N.; Vasilov, R.G.
Biulleten' Eksperimental'noi Biologii i Meditsiny 107(6): 722-724
1989
ISSN/ISBN: 0365-9615 PMID: 2676007 Document Number: 327371
Enzyme immunoassay for determination of total immunoglobulin E in children and adolescents based on combination of two monoclonal antibodies with high affinity and specificity to different epitopes of IgE molecule has been developed. Monoclonal antibodies IgE/11-4 are used for sensitization of a solid phase (96-well plate), mcAB IgE/11-1 conjugated with horseradish peroxidase are used as a developing reagent. The following analytical characteristics of a test-system are established: range of assay--1-100 IU/ml sensitivity--1 IU/ml; time of analysis--1.5 hour, sample volume in duplicates--30 microliters. Immunoassay is simple, reliable and can be used in the field of pediatrics and allergology.