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Immunoenzyme test-system for the detection of Vibrio cholerae 0139

Fedorova, V.A.; Gromova, O.V.; Devdariani, Z.L.

Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 5: 77-80

1998

ISSN/ISBN: 0372-9311
PMID: 9825508
Document Number: 489949
On the basis of antibodies to the lipopolysaccharide (LPS) and capsular antigen of V.cholerae O139 two variants of the enzyme immunoassay (EIA) system permitting the detection of the infective agent at a concentration of 1 x 10(6) microbial cells/ml were developed. Antibodies to V.cholerae O139 K-antigen and LPS were found to be highly active and specifically reacted only with homologous antigens without additional adsorption. Both variants of the EIA system, developed on the basis of antibodies to LPS or K-antigen, may be used for the detection of V.cholerae O139. The use of the latter variant making it possible to evaluate the virulence of the isolated cultures.

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