Vesiculation and change of erythrocyte membrane proteins in storage of preserved blood and erythrocyte mass at 4 degrees C
Chernitskiĭ, E.A.; Fedorovich, I.E.; Slobozhanina, E.I.
Gematologiia i Transfuziologiia 38(8): 18-21
1993
ISSN/ISBN: 0234-5730 PMID: 8307298 Document Number: 411935
Erythrocyte vesiculation and changes in membrane protein composition during storage of blood and red cell concentrates in solution "Glugicir" at 4 +/- 2 degrees C have been studied. It is shown that the main part of vesicles is shedding after 9 days of storage. This process corresponds to a decrease of protein 3 and to polyphase changes of membrane-bound hemoglobin as is shown by gel-electrophoresis. During 9-day storage, when erythrocyte ATP is constant and the vesicle shedding is small, an increase in membrane-bound hemoglobin and a decrease of spectrin in isolated membranes take place. The above changes are not the result of oxidative processes in membrane lipid bilayer or in hemoglobin. There was also protein destruction in membranes of stored erythrocytes. It is suggested that in stored blood two type of erythrocyte vesiculation take place: one is due to cell senescence, the other results from metabolic depletion.