Reticulin-M, an antianaphylactic peptide secreted by reticulo-endothelial system
Baciu, I.; Tomuş, L.; Cojocaru, A.; Felegean, A.; Albu, M.
Romanian Journal of Physiology 30(3-4): 183-192
1993
ISSN/ISBN: 0035-399X PMID: 7582931 Document Number: 411044
I. Moldovan (1923) demonstrated in the blood ultrafiltrate from anaphylactically prepared guinea pigs, which believed to anaphylactic shock, or which were previously injected with coloidal substances as India ink to stimulate the RES, an antianaphylactic principle named Reticulin-M(R). Authors extracted R from organs rich in RES with acetone, established their, peptidic nature and tested it on anaphylactic prepared guinea pig uterine horns in vitro. The peptides were fractioned by high voltage paper electrophoresis. The fractions were cut up in four groups, eluted and tested. The second basic group was identified as active and concentrated by acetone, recipitation. In conclusion several techniques were used to obtain, to test, to isolate and to concentrate R, a natural antianaphylactic peptidic factor, which may be a cytokine.