Screening for inhibitor of vascular endothelial growth factor from random peptide library
Wu, J.; Zhang, H.; Wang, J.; Yang, T.; Xian, J.; Yang, C.; Zheng, W.; Chen, H.; Wang, Q.
Zhonghua Zhong Liu Za Zhi 24(6): 540-543
2002
ISSN/ISBN: 0253-3766 PMID: 12667320 Document Number: 545702
To screen for the inhibitor of vascular endothelial growth factor (VEGF) 165 from random peptide library. Positive phage clones were rescued after two rounds of panning and competitive elution. Its affinity activity to KDR was monitored through ELISA, immunohistochemical method, Chicken CAM assay and MTT. Five specific binding positive target molecule phage clones were obtained which were able to bind to cells whose surface had high KDR, among which, clone 3 and 13 could effectively block the vascularization of the chorioallantoic membrane of chick embryo, but they were not inhibitive on the proliferation of high KDR expression cells. The peptides, being the inhibitors of VEGF, may be useful in the treatment of cancers.