Effects of Porphyromonas gingivalis on inflammatory factor secreted by human umbilical vein endothelial cells
Wang, X.-y.; Wang, J.-l.; Ding, A.; Sun, Y.; Wang, Q.-t.
Shanghai Kou Qiang Yi Xue 17(4): 395-399
2008
ISSN/ISBN: 1006-7248 PMID: 18784881 Document Number: 616511
To investigate the effect of porphyromonas gingivalis (P.g) on interleukin-18 (IL-18) and CRP secretion in human umbilical vein endothelial cells (HUVECs). Anaeropack method was used for P.g cultivation and then HUVECs were co-cultured with P.g followed by assaying the amount of IL-18 and CRP with enzyme-linked immunosorbent assay (ELISA) kit. Linear regression and variance analysis were performed with SPSS11.0 software package. The unstimulated HUVECs expressed small amounts of IL-18 and CRP, P.g dose-dependently increased the production of IL-18 and CRP in HUVECs. Elevated IL-18 and CRP were found at 4-hour, then continued to increase at 8-hour, 12-hour and 24-hour after co-cultivation; under the effect of same concentration, the quantity of CRP was significantly higher than that of IL-18 secreted by HUVECs. There was significant different, P<0.05. It is concluded that P.g increased the production of IL-18 and CRP in HUVECs in a dose and time dependent manner, elevated IL-18 and CRP may be involved in the regulation of inflammatory both in periodontal disease and formation of atherosclerotic plaque in cardiovascular disease(CVD).