Characterization of precipitating antibody in equine infectious anemia
Nakajima, H.; Kono, Y.; Ushimi, C.
Journal of Immunology 107(3): 889-894
1971
ISSN/ISBN: 0022-1767 PMID: 4328472 Document Number: 39792
Precipitating antibody was present in the gamma globulin fractionated from infected serum with ammonium sulfate and by DEAE cellulose chromatography. Antibody was also recovered in the 7S globulin fraction separated with Sephadex G-200 from serum samples collected both early and later in the disease. When 7S globulin was added to purified antigen, precipitation between the resulting antigen-globulin complex and a reference EIA antiserum was inhibited. Precipitating antibody was first detected shortly ater the first febrile reaction. At that time no neutralizing antibody was found, but the appearance of precipitating antibody coincided fairly well with that of the complement fixing (CF) antibody. In contrast to CF antibody, which usually disappeared shortly after onset of the disease, the precipitating antibody remained detectable for at least 150 days. The relationships of precipitating antibody to the CF and neutralizing antibodies are discussed. The value of the immunodiffusion reaction for diagnosis of EIA was discussed.