Use of Hoechst fluorescent probe 33258 for quantitative analysis of intracellular binding of anthracycline antibiotics to DNA
Egudina, S.V.; Baranov, E.P.; Bogush, T.A.; Kris'ko, S.I.
Antibiotiki i Khimioterapiia 37(5): 21-24
1992
ISSN/ISBN: 0235-2990 PMID: 1417323 Document Number: 389919
The paper deals with development of a procedure for quantitative determination of the share of anthracycline antibiotics bound in cells directly to DNA. A DNA-specific Hoechst fluorescence dye 33258 was used for the purpose. The level of its quenching on DNA correlated with the quantity of the antibiotic bound to it. It was shown that the quenching of the Hoechst fluorescence dye bound to DNA was not due to the dye competition with the antibiotic for the site of bounding on DNA, as was suggested earlier. It was likely to be defined by reabsorption of the radiation by antibiotic molecules.