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Application of fluorescent probe spectrum analysis for the investigation of the structural state of sorbent. Heterogeneous binding sites

Boĭtsov, V.M.; Orlov, S.N.

Biofizika 27(6): 1049-1052

1982

ISSN/ISBN: 0006-3029
PMID: 7159615
Document Number: 195687
A method is proposed for analyzing the fluorescence spectra of 1-anilinonaphthalene-8-sulphonate (ANS) and N-phenyl-1-naphthylamine (NPN) in the systems with nonuniform binding regions. Capacities of the method are demonstrated by analysing the fluorescence spectra of probes sorbed on bovine albumin molecules. It has been shown that there are two binding sites of the probes. Relative content of the probes in these binding sites was determined and their structural state was characterized (hydrophoby and microviscosity were estimated). The method described can be used to characterize the structural state of natural and artificial membranes.

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