An approach to screen nephrotoxic components in traditional Chinese medicine based on fluorescent probe and HK-2 cells and its application
Zhao, X.-P.; Guo, M.; Zhang, B.-L.
Zhongguo Zhong Yao Za Zhi 38(10): 1577-1580
2013
ISSN/ISBN: 1001-5302 PMID: 23947141 Document Number: 667150
Using fluorescein diacetate labeled HK-2 cells, a fast method for screening nephrotoxic components in traditional Chinese medicines (TCMs) was proposed in this study. The methodology validation showed that the linearity, stability and accuracy of the proposed method were suitable for screening nephrotoxic components in vitro . This method was further applied on screen 352 components from 32 Chinese Pharmacopoeia-indexed toxic TCMs. The results indicated that 31 components from 14 toxic TCMs, including Badou, had significant toxicity on HK-2 cells, which suggested these components may cause nephrotoxicity. The components from the other 18 toxic TCMs had no significant toxicity on HK-2 cells.