Top Abstract Purchase Related
Home
>
Node
>
Subnode

Analysis of the iron-binding sites of transferrin by isoelectric focussing

van Eijk, H.G.; van Noort, W.L.; Kroos, M.J.; van der Heul, C.

Journal of Clinical Chemistry and Clinical Biochemistry 16(10): 557-560

1978

ISSN/ISBN: 0340-076X
PMID: 30806
Document Number: 131130
Human transferrin was labeld with ferric nitrilotriacetate (FeNTA) at 1 of its 2 metal binding sites by variation of the pH. Four transferrin forms, transferrin, transferrin(Fe) (A-site), transferrin(Fe) (B-site) and transferrin(2Fe) were separated on flat bed gels by isoelectric focusing. Incubation time, temperature and medium play an important role in the specificity of the binding of Fe. In NTA-pH-buffer, at 0.5 Fe-saturation, the A-site was preferentially labeled at pH 7-8, the B-site at a pH 8-9. Under physiological conditions Fe from the B-site has the tendency to move to the A-site.

Document emailed within 1 workday
Secure & encrypted payments

Dudchenko, N.O.; Mykhaĭlik, O.M.; Lubianova, I.P. 2001: Effect of alpha-lipoic acid preparations on bilirubin and transferrin levels and ferritin iron and transferrin iron content Ukrains'kyi Biokhimichnyi Zhurnal 73(2): 102-105
Kämpfe, U.; Correns, A.; Schröder, H.; Otremba, P.; Geserick, G. 1991: Group-specific component imaging from trace material using isoelectric focussing and electroblotting Archiv für Kriminologie 187(3-4): 84-90
Celada, A.; Busset, R.; Gutiérrez, J.; Martínez, J.L.; Herreros, V. 1981: Comparative study of transferrin measurement and total iron binding capacity Sangre 26(6): 1073-1082
Engle-Stone, R.; Nankap, M.; Ndjebayi, A.O.; Erhardt, J.G.; Brown, K.H. 2013: Plasma ferritin and soluble transferrin receptor concentrations and body iron stores identify similar risk factors for iron deficiency but result in different estimates of the national prevalence of iron deficiency and iron-deficiency anemia among women and children in Cameroon Journal of Nutrition 143(3): 369-377
Lin, X-Ming.; Tian, W.; Ma, L.; Long, Z.; Zhang, J.; Shen, X-Yi.; Zhang, X-Peng. 2008: The responses of serum transferrin receptors to iron supplements in subjects with iron-deficiency erythropoiesis and iron-deficiency anaemia British Journal of Nutrition 99(2): 416-420
Ambrus, C.M.; Stadler, I.; Toumbis, C.A.; Stadler, A.; Anthone, S.; Anthone, R.; DeAlarcon, P.; Deshpande, G.; Conway, J.; Vladutiu, A.O.; Ambrus, J.L. 1999: Removal of non-transferrin-bound iron from blood with iron overload using a device with immobilized desferrioxamine Journal of Medicine 30(3-4): 211-224
Fillet, G.; Beguin, Y. 1989: Reutilization of iron in the body and regulation of iron reserves. the value of plasma transferrin receptor levels Bulletin et Memoires de l'Academie Royale de Medecine de Belgique 144(1-2): 113-118; Discussion: 118-120
Chen, Z.Z. 1984: Iron-deficiency in late pregnancy--observation on free erythrocyte protoporphyrin, transferrin saturation and marrow iron in 16 cases Zhonghua Yi Xue Za Zhi 64(2): 108-111
Borovina, T.A.; Nazipova, N.N.; Overbik, R.; Gel'fand, M.S. 1999: Statistical analysis and prediction of bacterial sites for ribosomal binding Biofizika 44(4): 611-619
Hou, C.C.; Wu, S.C.; Wu, S.C.; Chen, T.W.; Yang, W.C.; Ng, Y.Y. 1999: Is serum transferrin receptor a sensitive marker of iron repletion in patients with iron-deficiency anemia and hemodialysis patients? Zhonghua Yi Xue Za Zhi 62(4): 189-194
McCann, D.J.; Su, T.P. 1991: Solubilization and characterization of haloperidol-sensitive (+)-[3H]SKF-10,047 binding sites (sigma sites) from rat liver membranes Journal of Pharmacology and Experimental Therapeutics 257(2): 547-554
Sakurada, K.; Musashi, M.; Tanaka, J.; Matsuno, K. 1999: Total iron binding capacity (TIBC), unsaturated iron binding capacity (UIBC) Nihon Rinsho. Japanese Journal of Clinical Medicine 57 Suppl: 276-278
Boĭtsov, V.M.; Orlov, S.N. 1982: Application of fluorescent probe spectrum analysis for the investigation of the structural state of sorbent. Heterogeneous binding sites Biofizika 27(6): 1049-1052
Marcovich, C.E.; Marcovich, J.; Kersvenovich, D. 1976: Iron and transferrin metabolism Boletin Medico del Hospital Infantil de Mexico 33(3): 533-546
Olsen, R.W.; Bergman, M.O.; Van Ness, P.C.; Lummis, S.C.; Watkins, A.E.; Napias, C.; Greenlee, D.V. 1981: Gamma-Aminobutyric acid receptor binding in mammalian brain. Heterogeneity of binding sites Molecular Pharmacology 19(2): 217-227
Scotto di Tella, A.M.; Levy, C.; Lacarelle, B.; Tissot, R. 1986: Binding of tritiated imipramine to human platelets. 1 or several binding sites? L'Encephale 12(1): 13-17
DeGrella, R.F.; Coffee, E.E.; Combs, G.L. 1988: Effect of iron saturation on the TDx transferrin assay Clinical Chemistry 34(7): 1512
Banagale, R.C.; Bernstein, L.H. 1982: Reserve bilirubin binding capacity measured by absorbance deviation at saturation of primary albumin binding sites Research Communications in Chemical Pathology and Pharmacology 38(3): 463-479
Van Rossum, D.; Ménard, D.P.; Fournier, A.; St-Pierre, S.; Quirion, R. 1994: Autoradiographic distribution and receptor binding profile of [125I]Bolton Hunter-rat amylin binding sites in the rat brain Journal of Pharmacology and Experimental Therapeutics 270(2): 779-787
Mostafizar, M.; Haque, P.; Mazid, A.; Shohel, M.; Shazly, G.; Kazi, M.; Reza, H.M. 2017: Characterization of Binding Sites of Clopidogrel and Interference of Linoleic Acid at the Binding Site on Bovine Serum Albumin Acta Poloniae Pharmaceutica 74(1): 119-125