Chromosome aberration dosimetry: addition of thymidine, an interesting mean to increase the number of dividing cells
Dutrillaux, B.; Hilali, A.; Achkar, W.A.; Martins, B.; Sabatier, L.; Léonard, E.D.; Léonard, A.
Strahlentherapie und Onkologie Organ der Deutschen Rontgengesellschaft . 167(5): 319-321
1991
ISSN/ISBN: 0179-7158 PMID: 2038717 Document Number: 386818
Addition of thymidine to culture medium commonly used by laboratories performing medical cytogenetics followed by a release of the block by 2-deoxycitidine increases greatly the mitotic index and the proportion of metaphases suitable for chromosome analysis. The benefit is, however, less evident when the method is applied to culture medium used in laboratories specialized in chromosome aberration dosimetry.