Aberration of X chromosome in liver neoplasm detected by fluorescence in situ hybridization
Liu, J.; Wang, Z-Min.; Zhen, S-Fang.; Wu, X-Peng.; Ma, D-Xin.; Li, Z-Hui.; Liu, B.; Zhao, Z-Lun.; Ke, Y.
Hepatobiliary and Pancreatic Diseases International Hbpd Int 3(1): 110-114
2004
ISSN/ISBN: 1499-3872 PMID: 14969851 Document Number: 575391
A diverse range of cytogenetic alterations of autosomal chromosomes has been reported to date. However, few studies have addressed the abnormalities of X chromosome in hepatocellular carcinoma (HCC) except sporadic reports on the deletion of band F1 in X chromosome, and the clonal analysis of methylation pattern of the X chromosome-linked human androgen receptor gene. Identification of specific X chromosome alterations during the course of neoplastic development would be essential to defining the genetic basis of HCC. Therefore, we studied the regularity of aberration of X chromosome in liver cancer. Hepatocarcinoma cellular lines and tumor tissues were detected respectively through DNA probes of X chromosome after fluorescence in situ hybridization (FISH). Increased copies of X chromosome were observed in all samples, and four signals of hybridization were of the major type. Increased copy number of X chromosome frequently occur in liver cancer. The relationship between copy number of X chromosome and liver cancer genesis needs further investigation. This study is the first of its kind determining the copy number of X chromosome in liver cancer by using FISH.