The chromatography of yeast recombinant HBsAg in porous silicas
Kurbatova, I.I.; Krasil'nikov, I.V.; Borisova, V.N.; Zubov, S.V.
Voprosy Virusologii 35(4): 308-309
1990
ISSN/ISBN: 0507-4088 PMID: 2175066 Document Number: 355795
The results of the study of adsorption processes of recombinant HBsAg on various porous silicas are presented. The maximal yield of the recombinant antigen by absorption chromatography was observed on unmodified macroporous glass MPS-2000. A mean yield in this case was 85%. Silica C-80 modified with polyvinylpyrrolidone proved to be most acceptable for gel-penetrating chromatography. The adsorption and gel-penetrating chromatography on porous silicas proved to be an effective method for purification of recombinant HBsAg.
Document emailed within 1 workday
Related Documents
Shen, L.; Xiong, B.; Cong, R.; Wang, J. 1999: Cibacron blue F3GA-attached 2 microns non-porous monodisperse silicas for affinity chromatography Se Pu 17(5): 427-430Wirth, H.J.; Unger, K.K.; Hearn, M.T. 1991: High-performance liquid chromatography of amino acids, peptides and proteins. CIX. Investigations on the relation between the ligand density of cibacron blue immobilized porous and non-porous sorbents and protein-binding capacities and association constants Journal of Chromatography 550(1-2): 383-395
Sato, Y.; Ishikawa, N.; Takagi, T. 1990: High-performance size-exclusion chromatography and molar mass measurement by low-angle laser light scattering of recombinant yeast-derived human hepatitis B virus surface antigen vaccine particles Journal of Chromatography 507: 25-31
Qin, S.; Tang, H.; Zhao, L.; Liu, L.; Lin, Y. 2001: Construction of recombinant plasmids containing genes of HBsAg and their expression in the eukaryotic cells Hua Xi Yi Ke da Xue Xue Bao 32(2): 172-174
Brown, R.H. 1988: Determination of benzene, toluene and xylene in industrial air by porous polymer adsorption tube, thermal desorption and gas chromatography Iarc Scientific Publications 85: 235-242
D.Shah, O.; Coleman, P.; Chen, J.; Peterson, B.; Dimarco, A. and J. Stewart 2000: The Detection of Recombinant Hepatitis B Surface Antigen from "Vaccine Escape Mutants" in Two HBsAg Immunoassays Clinical Laboratory 46(3-4): 161-163
Kozhina, T.N.; Chepurnaia, O.V.; Fedorova, I.V. 1985: Recombinant plasmids carrying multiple markers: isolation during yeast co-transformation Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 5: 31-36
Onda, K.; Yang, S.Y.; Miya, A.; Tanaka, T. 2002: Evaluation of estrogen-like activity on sewage treatment processes using recombinant yeast Water Science and Technology: a Journal of the International Association on Water Pollution Research 46(11-12): 367-373
Shi, J.; Li, Z.; Zheng, L. 2001: Immunogenicity and safety of recombinant yeast-derived hepatitis B vaccine (YDV) on adults Zhonghua Liu Xing Bing Xue Za Zhi 22(2): 105-107
Moyes, C.; Milne, A. 1988: Immunogenicity of a recombinant yeast-derived hepatitis B vaccine (Engerix B) in children New Zealand Medical Journal 101(843): 162-164
Stasiuk, N.I.; Gaîda, G.Z.; Koval'chuk, I.P.; Stasyk, O.V.; Gonchar, M.V. 2010: Human arginase i from the recombinant yeast Hansenula polymorpha: isolation and characterization of the enzyme Ukrains'kyi Biokhimichnyi Zhurnal 82(6): 14-21
Antonopoulos, A.; Herbreteau, B.; Lafosse, M.; Helbert, W. 2004: Comparative analysis of enzymatically digested kappa-carrageenans, using liquid chromatography on ion-exchange and porous graphitic carbon columns coupled to an evaporative light scattering detector Journal of Chromatography A 1023(2): 231-238
Koziol, D.E.; Alter, H.J.; Kirchner, J.P.; Holland, P.V. 1976: The development of HBsAg-positive hepatitis despite the previous existence of antibody to HBsAg Journal of Immunology 117(6): 2260-2262
Hess, G.; Shih, J.W.; Arnold, W.; Gerin, J.L.; zum Büschenfelde, K.H. 1979: Demonstration and partial characterization of 22-nm HBsAg and Dane particles of subtype HBsAg/ady Journal of Immunology 123(3): 1189-1194
Frösner, G.G.; Deinhardt, F.; Scheid, R.; Kreiner, C.F.; Eisenburg, J. 1979: Detection of HBsAg and HBeAg in cleaning, rinsing and storage solutions of contact lenses and in tear fluid of carriers of HBsAg Klinische Monatsblatter für Augenheilkunde 175(4): 502-508
Longacre, S.; Mach, B. 1979: Purification of DNA or RNA sequences complementary to mercurated recombinant DNA by sulfhydryl sepharose chromatography Methods in Enzymology 68: 192-199
Lukhwareni, A.; Burnett, R.J.; Selabe, S.G.; Mzileni, M.O.; Mphahlele, M.J. 2009: Increased detection of HBV DNA in HBsAg-positive and HBsAg-negative South African HIV/AIDS patients enrolling for highly active antiretroviral therapy at a Tertiary Hospital Journal of Medical Virology 81(3): 406-412
Stevens, C.E.; Taylor, P.E.; Tong, M.J.; Toy, P.T.; Vyas, G.N.; Nair, P.V.; Weissman, J.Y.; Krugman, S. 1987: Yeast-recombinant hepatitis B vaccine. Efficacy with hepatitis B immune globulin in prevention of perinatal hepatitis B virus transmission JAMA 257(19): 2612-2616
Ratnayake, W.M.Nimal. 2004: Overview of methods for the determination of trans fatty acids by gas chromatography, silver-ion thin-layer chromatography, silver-ion liquid chromatography, and gas chromatography/mass spectrometry Journal - Association of Official Analytical Chemists 87(2): 523-539
Françoise Lunel-Fabiani; Duburcq, X.; Levayer, T.; Descamps, F.; Michèle Maniez-Montreuil; Pivert, A.; Ducancelle, A.; Pouzet, A.; Marant, L.; Cl, A.èment; Duhamel, D.; Leirens, Y.; Margotteau, F.; Falcou, R.-Briatte; Heinen, C.; Bouniort, F.; Taskar, S.; Artus, A.; Woodrum, D.; Flecheux, O. 2010: Multi-Center Evaluation of the Hepatitis B Surface Antigen (HBsAg) Assay and HBsAg Confirmatory Assay for the Family of Access® Immunoassay Systems Clinical Laboratory 56(7-8): 281-290