Comparative analysis of enzymatically digested kappa-carrageenans, using liquid chromatography on ion-exchange and porous graphitic carbon columns coupled to an evaporative light scattering detector
Antonopoulos, A.; Herbreteau, B.; Lafosse, M.; Helbert, W.
Journal of Chromatography A 1023(2): 231-238
2004
ISSN/ISBN: 0021-9673 PMID: 14753689 Document Number: 575712
Enzymatically digested kappa (A-G4S)-carrageenans, apart from their biological activities in plants, could be used as 'model' molecules to elucidate potential problems in nuclear magnetic resonance spectroscopy of carrageenans. Thus, oligosaccharides obtained from kappa-carrageenan by enzymatic digestion using kappa-carrageenase have been separated on silica and polymeric based ion-exchange and porous graphitic carbon (PGC) columns, coupled to an evaporative light scattering detector. Oligomers were separated on ion-exchange columns using a gradient of ammonium acetate as a developing ion, while analysis on PGC column presented an additional adjacent peak next to each main one, using a gradient of ammonium acetate in water/acetonitrile as a mobile phase. The phenomenon can be attributed to different retention mechanisms that govern the PGC surface. Furthermore, it has been demonstrated that acetonitrile can regulate the selectivity between the peaks raising hopes for preparative chromatography.