Determination of 5-hydroxytryptamine (5-HT) secretion from activated platelets by high performance liquid chromatography/electrochemical detector
Wang, L.; Guo, Z.; Zhao, D.; Mei, Q.
Se Pu 17(2): 223-224
1999
ISSN/ISBN: 1000-8713 PMID: 12549179 Document Number: 510927
A method of determination of 5-HT secretion from activated platelets by HPLC/ECD was established. Spherisorb C18 column of 4.6 mm i.d. x 100 mm was used in this experiment. The CV was 4.65%, The linear range was 0.002 5-1 mg/L. This method has the advantages of high speed, high sensitivity; specificity, of requiring a minimum of preparation of samples and of relatively low cost for instrumentation and reagents. It could reflect the activated situation of platelet induced by weak activator and be used in other biological sample.