Internally standardized method for the determination of nalbuphine in human plasma by means of high performance liquid chromatography with electrochemical coulometric detection
Wetzelsberger, N.; Lücker, P.W.; Erking, W.
Arzneimittel-Forschung 38(12): 1768-1771
1988
ISSN/ISBN: 0004-4172 PMID: 3245848 Document Number: 307899
A high performance liquid chromatographic method with internal analogue standardization and electrochemical detection for the determination of nalbuphine in human plasma is described. Using 3 ml plasma the lower limit of detection is below 50 pg/ml, during the routine assay the limit of determination can be fixed at about 250-300 pg/ml. The calibration curve is linear in the range between 0.163-65 ng/l, the recovery rate form plasma exceeds 80%. The method was successfully applied in several pharmacokinetic studies.