Human arginase i from the recombinant yeast Hansenula polymorpha: isolation and characterization of the enzyme
Stasiuk, N.I.; Gaîda, G.Z.; Koval'chuk, I.P.; Stasyk, O.V.; Gonchar, M.V.
Ukrains'kyi Biokhimichnyi Zhurnal 82(6): 14-21
2010
ISSN/ISBN: 0372-3909 PMID: 21805858 Document Number: 644664
Purified human arginase I preparations homogeneous in SDS-PAAG test were obtained by the affinity chromatography on the synthesized sorbent L-arginine-macroporous glass. Some physico-chemical characteristics of the isolated arginase preparation have been estimated: thermo- and pH-stability, temperature- and pH-optima of the enzyme. The influence of some bivalent metal ions and other additives on enzymatic activity for stabilization of the enzyme and optimization of its storage conditions was studied.