Top Abstract Purchase Related
Home
>
Node
>
Subnode

New reagents and solid support for automated oligonucleotide synthesis

Vu, H.; McCollum, C.; Lotys, C.; Andrus, A.

Nucleic Acids Symposium Series 22: 63-64

1990

ISSN/ISBN: 0261-3166
PMID: 2101916
Document Number: 353588
The optimal system for the rapid, efficient, convenient, and economical synthesis and purification of synthetic oligonucleotides has been advancing. By recognizing the very rapid reaction kinetics and taking advantage of an efficient, low volume delivery system, cycle times have decreased to about 5.5 minutes, without compromising synthesis performance. A new set of base protecting groups for cyanoethylphosphoramidite nucleoside monomers have been developed, which decreases the post-synthesis time requirements. A particular form of polystyrene has also been developed as a solid support for automated oligonucleotide synthesis. Typical sequencing or PCR primers (20mers) now require less than 2 hours for synthesis and 2 hours for cleavage and deprotection.

Document emailed within 1 workday
Secure & encrypted payments

Andrus, A.; Wright, P.; Wang, J.; Mullah, B.; Baier, J.; Mason, J.; Kaufman, G. 1995: Synthesis and purification in a single column on a high-throughput automated oligonucleotide production system Nucleic Acids Symposium Series 34: 183-184
Pon, R.T.; Buck, G.A.; Hager, K.M.; Naeve, C.W.; Niece, R.L.; Robertson, M.; Smith, A.J. 1996: Multi-facility survey of oligonucleotide synthesis and an examination of the performance of unpurified primers in automated DNA sequencing Biotechniques 21(4): 680-685
Jung, N.; Encinas, A.; Bräse, S. 2006: Automated synthesis of heterocycles on solid supports Current Opinion in Drug Discovery and Development 9(6): 713-728
Makarovskiĭ, V.V.; Biriukova, L.I.; Budkina, T.V.; Shagovikova, G.P. 1984: Automated biochemical laboratory. IV. Automated inventory system for chemical reagents Laboratornoe Delo 9: 528-533
Baru, M.B.; Cherskiĭ, V.V.; Danilov, A.V.; Moshnikov, S.A.; Mustaeva, L.G. 1995: The automated "SynChrom" system for solid-phase synthesis of peptides and liquid column chromatography. II. use in the solid-phase synthesis of peptides and liquid column chromatography Bioorganicheskaia Khimiia 21(7): 506-516
Driscoll, R.C.; Edwards, R.B.; Liston, M.D.; Love, T.W.; Mazza, J.C.; Trafton, J.E. 1983: Discrete automated chemistry system with tableted reagents Clinical Chemistry 29(9): 1609-1615
Curran, D.P. 1998: Fluorous synthesis: an alternative to organic synthesis and solid phase synthesis for the preparation of small organic molecules Cancer Journal from Scientific American 4(Suppl 1): S73-S76
Reese, C.B.; Song, Q. 1997: A new approach to oligonucleotide synthesis in solution Nucleic Acids Symposium Series 37: 1-2
Dubey IYa; Lyapina, T.V.; Fedoryak, D.M. 1991: H-phosphonate oligonucleotide synthesis in solution Nucleic Acids Symposium Series 24: 270
Vu, H.; Singh, P.; Joyce, N.; Hogan, M.E.; Jayaraman, K. 1993: Synthesis of cholesteryl supports and phosphoramidites containing a novel peptidyl linker for automated synthesis of triple-helix forming oligonucleotides (TFOs) Nucleic Acids Symposium Series 29: 19-20
Kitamura, M.; Nimura, A.; Kunimoto, K.; Segawa, H.; Shimidzu, T. 1991: Synthesis and properties of oligonucleotide derivative with P(V) porphyrin Nucleic Acids Symposium Series 25: 13-14
Miyoshi, K.; Itakura, K. 1980: Solid-phase synthesis of polynucleotides: V. Synthesis of oligodeoxyribonucleotides by the phosphomonotriazolide method Nucleic Acids Symposium Series 7: 281-291
Kamaike, K.; Yamakage, S.; Hasegawa, Y.; Ishido, Y. 1986: Oligonucleotide synthesis on a cellulose acetate functionalized with a spacer Nucleic Acids Symposium Series 17: 89-92
Takaku, H.; Yoshida, M.; Hata, T. 1979: 8-Quinolinesulphonyltetrazolide (QStet) as a new coupling agent for oligonucleotide synthesis via phosphotriester approach Nucleic Acids Symposium Series 6: S181-S182
Almog, J.; Hirshfeld, A.; Klug, J.T. 1982: Reagents for the chemical development of latent fingerprints: synthesis and properties of some ninhydrin analogues Journal of Forensic Sciences 27(4): 912-917
Pontén, I.; Kim, S.K.; Gräslund, A.; Nordén, B.; Jernström, B. 1994: Synthesis and characterization of a (+)-anti-benzo[a]pyrene 7,8-dihydrodiol 9,10-epoxide-oligonucleotide adduct Iarc Scientific Publications 1994(125): 433-436
Wada, T.; Tobe, M.; Nagayama, T.; Furusawa, K.; Sekine, M. 1993: New strategies for oligonucleotide synthesis by use of 2-trimethylsilylethyl and 2-trimethylsilylethoxymethyl as the phosphate and 2'-hydroxyl protecting groups, respectively Nucleic Acids Symposium Series 29: 9-10
Denisova, L.I.; Zagrebel'nyĭ, S.N.; Pustoshilova, N.M. 1980: Reiterative poly(A) synthesis on oligonucleotide templates in an Escherichia coli RNA-polymerase system Molekuliarnaia Biologiia 14(6): 1372-1377
Hutzinger, O.; Jamieson, W.D.; Macneil, J.D.; Frei, R.W. 1971: Electron-donor-acceptor complexing reagents for the analysis of pesticides. I. Survey of reagents and instrumental techniques Journal - Association of Official Analytical Chemists 54(5): 1100-1109
Korshunova, G.A.; Sumbatian, N.V.; Topin, A.N.; Mchedlidze, M.T. 2000: Photoactivated reagents based on aryl(trifluoromethyl)diazirines: synthesis and use for studying nucleic acid-protein interactions Molekuliarnaia Biologiia 34(6): 966-983