Use of Streptomyces sp. 26-115 protoplasts inactivated by heating in fusion experiments
Orlova, T.I.; Kuroedov, A.A.; Volkova, E.I.
Antibiotiki i Khimioterapiia 34(2): 94-98
1989
ISSN/ISBN: 0235-2990 PMID: 2730229 Document Number: 339462
When heated at 55 degrees C for 30 or 60 minutes protoplasts of auxotrophic mutants of Streptomyces sp. 26-115 producer of actinomycin C (active and inactive variants) lost their capacity for regeneration. The protoplasts heated at at 55 degrees C for 30 minutes and not for 60 minutes maintained some ability to yield recombinants on fusion under the effect of PEG 6000. Unlike the parent active strain, the colonies formed by the spores of the prototrophs yielding on fusion of the intact protoplasts showed wide ranges of antibiotic activity against M. flavus while a significant part of the colonies was inactive. The use of the inactive variant protoplasts heated at 55 degrees C for 30 minutes in the fusion procedure increased the proportion of the inactive variants.