Isolation and regeneration of Streptomyces griseus protoplasts
Sten'ko, A.S.; Bezkorovaĭnaia, N.K.
Mikrobiolohichnyi Zhurnal 45(2): 29-33
1983
ISSN/ISBN: 0201-8462 PMID: 6408362 Document Number: 220454
A hypertonic salt soy-based medium with osmotic stabilizers, Mg (10 mM) and CA (25 mM) was optimal for producing stable protoplasts of S. griseus strain 68-31. The effective regeneration into mycelial forms occurred in a medium of the same composition with 20 mM Mg and 25 mM Ca.
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