Solvent systems for thin layer chromatography of biological dyes
Allison, R.T.; Garratt, N.J.
Medical Laboratory Sciences 46(2): 113-119
1989
ISSN/ISBN: 0308-3616 PMID: 2593771 Document Number: 331469
Thirty-nine dyes were subjected to thin layer chromatography to evaluate eight different solvent systems. The results obtained were analyzed by dye class following assessment in daylight and ultra violet light. N-butanol: acetic acid: water (40:10:50) gave optimum separations of dyes viewed in visible light. Although n-butanol: ethanol: water (90:10:10) gave slightly better results for fluorescing compounds, material was frequently left at the start point with this solvent.