Thin-layer chromatography of apomorphine and its analogs
Erhardt, P.W.; Smith, R.V.; Sayther, T.T.; Keiser, J.E.
Journal of Chromatography 116(1): 218-224
1976
ISSN/ISBN: 0021-9673 PMID: 173731 Document Number: 102648
As part of systematic studies concerning the mammalian metabolism of aporphine alkaloids, recently the metabolic fate of apomorphine analogs which are potential anti-parkinsonian prodrugs was studied. Preliminary investigation indicated that significant decomposition of apomorphine (I) and aporphines occurs during typical microsomal metabolism studies, e.g., during both microsomal incubation yields single, characteristic spots for each of the compounds. TLC solvent systems were developed to separate the metabolic substrates II-VIII from I and/or other probable metabolites. These systems and structural characterization of the major decomposition product of I are described.