Ultrastructure, peroxisomes and lipid peroxidation in reperfused myocardium
Vaughan, D.M.; Koke, J.R.; Bittar, N.
Cytobios 55(221): 71-80
1988
ISSN/ISBN: 0011-4529 PMID: 3229146 Document Number: 315897
Reperfusion injury was studied in dog myocardium using a transmission electron microscope and 3,3'-diaminobenzidine (DAB) to locate areas of peroxidatic activity. Dark electron dense DAB reaction product was observed in peroxisomes and damaged mitochondria. These results suggest attack by reactive oxygen species on mitochondrial membranes, which might result in the formation of lipid peroxides and prostaglandin-like compounds. It is suggested that the release of lipid peroxide or prostaglandins from the injured cells may contribute to reactive hyperaemia, ventricular fibrillation and angina.