The sensitivity of guinea pig mesenteric and pulmonary mast cells to some histamine releasers
Brezezińska-Błaszczyk, E.; Czuwaj, M.; Wyczółkowska, J.
Archivum Immunologiae et Therapiae Experimentalis 35(6): 871-876
1987
ISSN/ISBN: 0004-069X PMID: 2468320 Document Number: 301724
The mesenteric and pulmonary mast cells of guinea pigs were obtained by enzymatic dispersion of the tissues with the enzyme collagenase. The guinea pig mast cells obtained by this method were morphologically intact, as judged by light microscopy. The mast cells from lung and mesentery of actively sensitizied guinea pigs released histamine in a dose-dependent fashion after challenge with specific antigen. Both pulmonary and mesenteric mast cells of nonsensitized animals were unresponseive to the action of compound 48/80 and concanavalin A. Both populations of mast cells responded to polymyxin B, but the magnitude of histamine release was low. These cells responded strongly to the inophore A23187; the mesenteric mast cells were markedly more reactive than pulmonary mast cells.