The mite allergen and allergoid stimulation of histamine secretion by mast cells
Khlgatian, S.V.; Perova, N.A.; Berzhets, V.M.
Biulleten' Eksperimental'noi Biologii i Meditsiny 116(9): 311-313
1993
ISSN/ISBN: 0365-9615 PMID: 7509651 Document Number: 414507
Rat peritoneal mast cells were incubated with serum from highly mite-sensitive patients. It was demonstrated that exposure of passive sensitized mast cells to allergen from mites Dermatophagoides farinae induced the release of histamine. Exposure of mast cells to 10 micrograms/ml and 50 micrograms/ml mite allergen resulted in an increase of histamine secretion to 48% of the basal level. The allergoid (formaldehyde-modified mite allergen) had poor histamine-releasing activity compared to allergen. The allergoid (50 micrograms/ml) induced a 2.5-fold decrease in histamine release. The allergen at the same concentrations and the same release as allergen in dose 0.1 microgram/ml.