Top Abstract Purchase Related
Home
>
Node
>
Subnode

Isolation and use in virological research of a peroxidase conjugate with protein a

Zvonarev, A.I.; Mal'tseva, N.N.

Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 9: 85-87

1987

ISSN/ISBN: 0372-9311
PMID: 2825453
Document Number: 301357
On the basis of proteins A produced at the Mechnikov Central Research Institute for Vaccines and Sera (Moscow) and obtained from Pharmacia AB (Sweden), several batches of peroxidase conjugate have been prepared by a modified method of periodate oxidation. The preparations thus obtained have been evaluated by means of the corresponding enzyme immunoassay systems for the detection of antibodies to herpes simplex and measles viruses. The results of this investigation indicate that serum antibody titers, determined in the assays with antispecific conjugates and with protein A-based based conjugates, coincide. The comparative study of conjugates prepared on the basis of Soviet and imported proteins A has demonstrated their similar activities and specificities.

Document emailed within 1 workday
Secure & encrypted payments

Egorenkova, E.M.; Khristova, M.L.; Leonov, S.V.; Kozhukharova, M.; Demidova, S.A. 1987: Use of immunoenzyme analysis employing a staphylococcal protein a conjugate with peroxidase in the serodiagnosis of influenza Voprosy Virusologii 32(2): 168-172
Vinskaia, A.I.; Grigorashkin, I.I. 1992: The quantitative detection of orthopoxvirus antibodies in studying sera in a single dilution in an immunoenzyme test system with a conjugate of protein a and peroxidase Voprosy Virusologii 37(2): 124-127
Yu, L.; Liu, J.H.; Shao, M.F.; Li, X.M.; Zhang, X.M. 1988: An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against swine fever virus using horseradish-peroxidase-protein-A as conjugate Deutsche Tierärztliche Wochenschrift 95(3): 106-107
Vella, P.P.; Staub, J.M.; Armstrong, J.; Dolan, K.T.; Rusk, C.M.; Szymanski, S.; Greer, W.E.; Marburg, S.; Kniskern, P.J.; Schofield, T.L. 1990: Immunogenicity of a new Haemophilus influenzae type b conjugate vaccine (meningococcal protein conjugate) (PedvaxHIB) Pediatrics 85(4 Part 2): 668-675
Yasuda, K.; Yamamoto, N.; Yamashita, S. 1981: Use of peroxidase-avidin conjugate for the demonstration of intracellular antigen Experientia 37(3): 306-308
Volina, E.G.; Ivanov, A.P.; Kiktenko, V.S.; Tkachenko, E.A.; Arutiunova, G.A. 1986: The use of direct immunoenzyme analysis with genus-specific peroxidase conjugate in leptospirosis Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 12: 72-75
Käyhty, H.; Peltola, H.; Eskola, J.; Rönnberg, P.R.; Kela, E.; Karanko, V.; Mäkelä, P.H. 1989: Immunogenicity of Haemophilus influenzae oligosaccharide-protein and polysaccharide-protein conjugate vaccination of children at 4, 6, and 14 months of age Pediatrics 84(6): 995-999
Ghetie, M.A.; Moraru, I.; Margineanu, M.; Ghetie, V. 1988: Protein A vectorized toxins--II. Preparation and "in vitro" cytotoxic effect of protein A-ricin A chain conjugate on antibody coated human tumour cells Molecular Immunology 25(5): 473-477
Gouyette, A.; Voisin, E.; Auclair, C.; Paoletti, C. 1987: Isolation and characterization of the glutathione-elliptinium conjugate in human urine Anticancer Research 7(4b): 823-827
Hignite, C.E.; Tschanz, C.; Huffman, D.H.; Azarnoff, D.L. 1978: Quantitation of N-demethylantipyrine in biological samples and isolation and characterization of its glucuronic acid conjugate Drug Metabolism and Disposition: the Biological Fate of Chemicals 6(3): 288-295
Kolosova, L.V.; Kim, B.B.; Cherednikova, T.V.; Sadvanova, G.G.; Gavrilova, E.M. 1988: Isolation and characterization of monoclonal antibodies to horseradish peroxidase Biokhimiia 53(11): 1858-1863
Dzantiev, B.B.; Gavrilova, E.M.; Filippova, N.I.; Egorov, A.M.; Berezin, I.V. 1978: Isolation and properties of human immunoglobulin-peroxidase complex Biokhimiia 43(6): 1054-1061
Terletskaia, I.T.; Syrovatskaia, L.P.; Khzuliná, E.P.; Ovander, M.N.; Belik, I.V. 1980: Binding of protein SCP to myelin, its identity with protein P2. a simple method of protein P2 isolation Ukrainskii Biokhimicheskii Zhurnal 52(6): 753-758
Faerman, A.I.; Chervonskiĭ, A.V.; Chipysheva, T.A.; Gel'shteĭn, V.I.; Koliada, A.I. 1987: Isolation of monoclonal antibodies to horseradish peroxidase and their use in immunohistochemistry and immunoblotting Biulleten' Eksperimental'noi Biologii i Meditsiny 103(5): 631-634
Somov, G.P. 1983: Virological research in the eastern USSR Vestnik Akademii Meditsinskikh Nauk SSSR 12: 82-87
Meyer, M.; Gahr, M. 1993: Immunological principles of polysaccharide-protein conjugate vaccination Monatsschrift Kinderheilkunde: Organ der Deutschen Gesellschaft für Kinderheilkunde 141(10): 770
Divizia, M.; Panà, A. 1999: The applicability of molecular biology in environmental virological research Annali di Igiene: Medicina Preventiva e di Comunita 11(6): 461-466
Mordovtsev, V.N.; Starkov, I.V.; Zabarovskiĭ, E.R.; Chumakov, I.M.; Stepanova, L.V. 1987: Psoriasis: electron microscopic, virological and molecular biology research Vestnik Dermatologii i Venerologii 7: 4-10
Giuliani, G.; Paggi, G.C.; Faccioni, P.; Novara, O.; Varetti, G.; Gandolfo Caramello, M.T.; Gagliardi, L.; Fogli, L. 1977: Research on the rubella epidemic of 1973 in Turin. Virological data Bollettino dell'Istituto Sieroterapico Milanese 56(2): 164-170
Sorof, S.; Young, E.M. 1973: The principal liver carcinogen-protein conjugate after a single dose of hepatic azocarcinogen Cancer Research 33(9): 2010-2013