Isolation and properties of human immunoglobulin-peroxidase complex
Dzantiev, B.B.; Gavrilova, E.M.; Filippova, N.I.; Egorov, A.M.; Berezin, I.V.
Biokhimiia 43(6): 1054-1061
1978
ISSN/ISBN: 0320-9725 PMID: 96877 Document Number: 125430
The complexes of human immunoglobulin G (IgG) with peroxidase produced by a two-step synthesis using glutaraldehyde retain high enzymatic and immunochemical activities. The effectiveness of the complex formation depends on the concentration of glutaraldehyde used for enzyme modification. Optimal molar ratios of the constituent components during the synthesis of the complex and localization of the enzyme on the IgG molecule are discussed. Preliminary heating of IgG or the overall complex at 50 degrees C results in an increase of the complex stability (20-fold) under storage.