Isolation and properties of 2 kallikreins from human saliva
Vovchuk, S.V.; Barabash, R.D.; Levitsky, A.P.
Voprosy Meditsinskoi Khimii 21(6): 622-625
1975
ISSN/ISBN: 0042-8809 PMID: 3025 Document Number: 93485
Two kallikreins (K-I and K-II) were purified from mixed, parotid and submandibular human saliva. The kallikreins were separated by chromatography on CM-cellulose. The pH optima of activity were at pH 9.3 and pH 9.6-9.8; Km for BAEE was 1.10-3M and 4.10-3M, respectively. The esterase activity of K-I and K-II was inhibited by trasylol-like inhibitors, while the plant and synthetic inhibitors of trypsin were uneffective. In dog, rabbit and rat the hypotensive effect of K-II and its action upon the permeability of rabbit skin was 4-5 fold higher than the effects of K-I. Ki and K-II did not alter the arterial blood pressure in guinea pig.