Use of peroxidase-avidin conjugate for the demonstration of intracellular antigen
Yasuda, K.; Yamamoto, N.; Yamashita, S.
Experientia 37(3): 306-308
1981
ISSN/ISBN: 0014-4754 PMID: 7016576 Document Number: 181941
Horseradish peroxidase was coupled to avidin by periodate oxidation. Using the peroxidase-avidin conjugate together with biotin-labeled anti-chymotrypsinogen pancreas. The reaction between avidin and biotin is so rapid and stable that the avidin-biotin system can be effectively applied to immunohistochemical studies.
Document emailed within 1 workday
Related Documents
Gallois, Y.; Tricaud, A.; Foussard, F.; Dubremetz, J.F.; Hodbert, J.; Girault, A.; Mauras, G. 1986: Toxoplasma gondii: demonstration of intrinsic peroxidase activity during lacto-peroxidase mediated radioiodination of tachyzoites Biomedicine and PharmacoTherapy 40(3): 111-113Yin, Q.Z.; Ye, M.J.; Xu, X.; Liu, H.C.; Liu, J.; Liu, X.M. 1988: The use of avidin-biotin peroxidase complex-ELISA for the examination of HBsAg Hua Xi Yi Ke da Xue Xue Bao 19(2): 174-176
Happerfield, L.C.; Bobrow, L.G.; Bains, R.; Miller, K.D. 1993: Peroxidase labelling immunocytochemistry: a comparison of eleven commercially-available avidin-biotin systems British Journal of Biomedical Science 50(1): 21-26
Kang, Y.S.; Boado, R.J.; Pardridge, W.M. 1995: Pharmacokinetics and organ clearance of a 3'-biotinylated, internally [32P]-labeled phosphodiester oligodeoxynucleotide coupled to a neutral avidin/monoclonal antibody conjugate Drug Metabolism and Disposition: the Biological Fate of Chemicals 23(1): 55-59
Kang, Y.S.; Bickel, U.; Pardridge, W.M. 1994: Pharmacokinetics and saturable blood-brain barrier transport of biotin bound to a conjugate of avidin and a monoclonal antibody to the transferrin receptor Drug Metabolism and Disposition: the Biological Fate of Chemicals 22(1): 99-105
Shigemoto, K.; Hamaguchi, N.; Okushin, S.; Hirabayashi, A.; Kobayashi, M.; Arita, M.; Usui, K.; Wada, K.; Yorioka, N.; Yamakido, M. 1987: Study on IgA, IgA subclass and complement in the glomerulus of IgA nephropathy by the avidin-biotin-peroxidase complex method Nihon Jinzo Gakkai Shi 29(3): 261-270
Charpin, C.; Martin, P.M.; Lissitzky, J.C.; Lavaut, M.N.; Kopp, F.; Pourreau-Schneider, N.; Jacquemier, J.; Toga, M. 1985: Immunocytochemical antigens detection in human breast carcinomas: a light and electron microscopy study using avidin biotin peroxidase and preembedding method Cancer Detection and Prevention 8(1-2): 77-85
Zvonarev, A.I.; Mal'tseva, N.N. 1987: Isolation and use in virological research of a peroxidase conjugate with protein a Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 9: 85-87
Volina, E.G.; Ivanov, A.P.; Kiktenko, V.S.; Tkachenko, E.A.; Arutiunova, G.A. 1986: The use of direct immunoenzyme analysis with genus-specific peroxidase conjugate in leptospirosis Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 12: 72-75
Egorenkova, E.M.; Khristova, M.L.; Leonov, S.V.; Kozhukharova, M.; Demidova, S.A. 1987: Use of immunoenzyme analysis employing a staphylococcal protein a conjugate with peroxidase in the serodiagnosis of influenza Voprosy Virusologii 32(2): 168-172
Peters, J.; Schmidt-Gayk, H.; Peters, B.; Armbruster, F.P.; Quentmeier, A.; Mathias, D. 1989: Immunoradiometric assay of carcinoembryonic antigen with use of avidin-biotin labeling Clinical Chemistry 35(4): 573-576
Calafat, J.; Molthoff, C.; Janssen, H.; Hilkens, J. 1988: Endocytosis and intracellular routing of an antibody-ricin a chain conjugate Cancer Research 48(13): 3822-3827
Vinskaia, A.I.; Grigorashkin, I.I. 1992: The quantitative detection of orthopoxvirus antibodies in studying sera in a single dilution in an immunoenzyme test system with a conjugate of protein a and peroxidase Voprosy Virusologii 37(2): 124-127
Yu, L.; Liu, J.H.; Shao, M.F.; Li, X.M.; Zhang, X.M. 1988: An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against swine fever virus using horseradish-peroxidase-protein-A as conjugate Deutsche Tierärztliche Wochenschrift 95(3): 106-107
Breton-Gorius, J.; Guichard, J. 1976: Improved methods for the cytochemical demonstration of platelet peroxidase Nouvelle Revue Francaise d'Hematologie 16(3): 381-389
Nakane, P.K. 1971: Application of peroxidase-labelled antibodies to the intracellular localization of hormones Acta Endocrinologica. Supplementum 153: 190-204
Strutz, J. 1987: Anatomy of the central auditory pathway. Demonstration with horseradish peroxidase in the guinea pig Hno 35(10): 407-415
Senyk, G.; Michaeli, D. 1973: Induction of cell-mediated immunity and tolerance to homologous collagen in guinea pigs: demonstration of antigen-reactive cells for a self-antigen Journal of Immunology 111(5): 1381-1388
Morenkov, O.S.; Mantsygin, I.A. 1990: The demonstration of proliferating cells by using monoclonal antibodies to 5-bromo-2'-deoxyuridine in the peroxidase-antiperoxidase method Tsitologiia 32(12): 1225-1230
Wollweber, L. 1975: The use of an ovomucoid-peroxidase complex for ultracytochemical demonstration of wheat germ agglutinin membrane receptors Acta Histochemica 54(2): 335-338