Synthesis, cloning and determination of the primary structure of DNA complementary to mRNA of prolactin from the human pituitary gland
Mertvetsov, N.P.; Golovin, S.Ia.; Zelenin, S.M.; Morozova, T.V.; Karginov, V.A.
Bioorganicheskaia Khimiia 13(12): 1687-1690
1987
ISSN/ISBN: 0132-3423 PMID: 3450284 Document Number: 300218
The poly(A)-containing mRNA from human pituitary and prolactinoma have been purified and translated in the cell-free system from rabbit reticulocytes. mRNA from prolactinoma was shown to be enriched with specific prolactin mRNA. DNA complementary to the prolactin mRNA from human pituitary was obtained and cloned. Sequencing of the 900 bp insert by the Maxam-Gilbert technique suggested the cDNA cloned to cole for the previously published amino acid sequence, mismatches with mRNA from prolactinoma occurring at the third positions of codons and thus not causing amino acid substitutions.