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Cloning and determination of the primary structure of DNA complementary to the mRNA of human ribosomal protein L11

Mishin, V.P.; Filipenko, M.L.; Muravlev, A.I.; Karpova, G.G.; Mertvetsov, N.P.

Bioorganicheskaia Khimiia 21(2): 158-160

1995

ISSN/ISBN: 0132-3423
PMID: 7748210
Document Number: 440776
A polymerase chain reaction strategy was employed to isolate cDNA encoding L11 human ribosomal protein. Based on the known nucleotide sequence of 5'-region of the ribosomal protein L11 mRNA, we have designed primers and used them in amplification of corresponding sequence of human cDNA from total placenta cDNA. The fragment of RPS26 cDNA was cloned in plasmid vector and sequenced. Sequence analysis showed that there is high homology (88%) between coding regions of RPS26 mRNAs in rat liver and human placenta. The amino acid exchanges were observed at positions: 91 (Asp-->Glu), 217 (Thr-->Ala), 352 (Lys-->Glu).

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