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Amplification using one-sided polymerase chain reaction, cloning and determination of primary structure of 5'-untranslated cDNA segments coding the somatogenic growth hormone receptor from rat liver

Pekhletskiĭ, R.I.; Chernov, B.K.; Rubtsov, P.M.

Molekuliarnaia Biologiia 25(5): 1418-1423

1991

ISSN/ISBN: 0026-8984
PMID: 1753966
Document Number: 385369
The method of ligation-mediated, single-sided polymerase chain reaction was applied for the amplification of 5'-untranslated regions of cDNA coding for somatogenic growth hormone receptor from rat liver. The products of polymerase chain reaction were cloned and sequenced. Two variants of 5'-untranslated sequences of growth hormone receptor cDNA corresponding to products of alternative splicing of pre-mRNA were found.

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