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Structure of nuclear pre-mRNA. VIII. Isolation and characterization of complementary sequences

Ryskov, A.P.; Enikolopov, G.N.; Limborskaya, S.A.

Molekuliarnaia Biologiia 10(4): 748-755

1976

ISSN/ISBN: 0026-8984
PMID: 1023053
Document Number: 108554
After the annealing of pre-mRNA at high Cot values, up to 20% of the material becomes resistant to the action of ribonuclease. This has been attributed to the presence of self-complementary sequences (scRNA) in the pre-mRNA. The most important properties of scRNA, which was isolated in preparative amounts, have been studied. The approximate dimensions of the complementary segments are 45-50 nucleotides. Hybridization experiments have shown that scRNA is transcribed from repeated segments of the genome. It may be postulated that the rapidly hybridizing pre-mRNA fraction consists mainly of self-complementary sequences.

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Strub, K.; Galli, G.; Busslinger, M.; Birnstiel, M.L. 1984: The cDNA sequences of the sea urchin U7 small nuclear RNA suggest specific contacts between histone mRNA precursor and U7 RNA during RNA processing EMBO Journal 3(12): 2801-2807
Devin, A.B.; Koltovaia, N.A.; Gavrilov, B.V.; Arman, I.P. 1994: Isolation and characterization of new nuclear srm gene mutation causing coordinated changes in the maintenance of nuclear and mitochondrial genetic structures in the yeast Saccharomyces Genetika 30(9): 1194-1201
Mishin, V.P.; Filipenko, M.L.; Muravlev, A.I.; Karpova, G.G.; Mertvetsov, N.P. 1995: Cloning and determination of the primary structure of DNA complementary to the mRNA of human ribosomal protein L11 Bioorganicheskaia Khimiia 21(2): 158-160
Erttmann, K.D.; Meredith, S.E.; Greene, B.M.; Unnasch, T.R. 1990: Isolation and characterization of form specific DNA sequences of O. volvulus Acta Leidensia 59(1-2): 253-260
Mertvetsov, N.P.; Golovin, S.Ia.; Zelenin, S.M.; Morozova, T.V.; Karginov, V.A. 1987: Synthesis, cloning and determination of the primary structure of DNA complementary to mRNA of prolactin from the human pituitary gland Bioorganicheskaia Khimiia 13(12): 1687-1690
Hirano, T.; Funahashi, S.; Uemura, T.; Yanagida, M. 1986: Isolation and characterization of Schizosaccharomyces pombe cutmutants that block nuclear division but not cytokinesis EMBO Journal 5(11): 2973-2979
Marzona, M. 1974: Structure of the keratin of psoriasic squamae. II. Isolation and characterization of a pure protein component Bollettino della Societa Italiana di Biologia Sperimentale 50(20): 1658-1661
Tanaka, T.; Kurihara, Y.; Sakamoto, T.; Furusawa, M.; Kobayashi, A.; Katahira, M.; Takeuchi, K.; Sugimoto, N.; Uesugi, S. 1995: Structure of translation initiation region of c-myc mRNA and thermostability of the c-myc mRNA/antisense ODN complex Nucleic Acids Symposium Series 1995(34): 135-136
Mertvetsov, N.P.; Golovin, S.Ia.; Beklemishev, A.B.; Karginov, V.A.; Mamaev, L.V. 1987: Cloning of DNA complementary to mRNA for proopiomelanocortin from the bovine, rat and human hypophysis. Hormonal regulation of proopiomelanocortin mRNA in the rat hypophysis Biokhimiia 52(5): 707-714
Yazdi, E.; Györkey, F.; Busch, H.; Györkey, P. 1971: Biochemical study of nuclei isolated from normal lung and lung tumors. I. Isolation of nuclei and characterization of nuclear RNA Journal of the National Cancer Institute 47(1): 121-129
Hoeben, R.C.; Fallaux, F.J.; Schagen, F.H.; van der Eb, M.M.; van Ormondt, H.; van der Eb, A. 1997: Factors impeding efficient expression of factor VIII complementary DNA minigenes Blood Coagulation and Fibrinolysis: An International Journal in Haemostasis and Thrombosis 8 Suppl. 2: S15-S21
Longacre, S.; Mach, B. 1979: Purification of DNA or RNA sequences complementary to mercurated recombinant DNA by sulfhydryl sepharose chromatography Methods in Enzymology 68: 192-199
Mahasandana, C.; Suvatte, V.; Püng-amritt, P.; Tuchinda, S. 1979: Von Willebrand's disease: studies of platelet functions, factor VIII procoagulant activity (F VIII C) and factor VIII-related antigen (F VIII RAg) in three families Southeast Asian Journal of Tropical Medicine and Public Health 10(2): 243-247
Dominski, Z.; Kole, R. 1996: Effects of exon sequences on splicing of model pre-mRNA substrates in vitro Acta Biochimica Polonica 43(1): 161-173
Romig, H.; Fackelmayer, F.O.; Renz, A.; Ramsperger, U.; Richter, A. 1992: Characterization of SAF-A, a novel nuclear DNA binding protein from HeLa cells with high affinity for nuclear matrix/scaffold attachment DNA elements EMBO Journal 11(9): 3431-3440
Ill, C.R.; Yang, C.Q.; Bidlingmaier, S.M.; Gonzales, J.N.; Burns, D.S.; Bartholomew, R.M.; Scuderi, P. 1997: Optimization of the human factor VIII complementary DNA expression plasmid for gene therapy of hemophilia A Blood Coagulation and Fibrinolysis: An International Journal in Haemostasis and Thrombosis 8 Suppl. 2: S23-S30
Gorodetskiĭ, S.I.; Kiarshulite, D.R.; Ivanov, V.N.; Kapelinskaia, T.V.; Kaledin, A.S. 1985: Synthesis and cloning of DNA complementary to mRNA of the bovine mammary gland Genetika 21(2): 191-200
Zharkikh, A.A.; Solov'ev, V.V.; Kolchanov, N.A. 1985: The context analysis of polynucleotide sequences. II. Inverted repeats and complementary palindromes in RNA-polymerase genes Molekuliarnaia Biologiia 19(3): 597-609
Benimetskaia, L.Z.; Gerasimova, L.M.; Karpova, G.G.; Krutilina, A.I.; Grineva, N.I. 1979: Specific fragmentation of phage lambda DNA in the vicinity of the 5'-ends of the oligothymidylic sequences by alkylation in the complementary regions Biokhimiia 44(5): 803-810
Alakhov, I.B.; Bundule, M.A.; Bundulis, I.P.; Vinokurov, L.M.; Kozlov, V.P. 1983: Primary structure of the elongation factor G from Escherichia coli. VIII. Structure of tryptic peptides comprising the T4 fragment of limited trypsinolysis of the G-factor Bioorganicheskaia Khimiia 9(3): 330-342