The synthesis of peptides with potential thymic hormone activity: the synthesis of endo-Arg38a-deacetylthymosin beta 10
Abiko, T.; Sekino, H.
Biotechnology and Applied Biochemistry 9(1): 20-30
1987
ISSN/ISBN: 0885-4513 PMID: 3494463 Document Number: 296673
A tritetracontapeptide corresponding to the entire amino acid sequence of endo-Arg38a-deacetylthymosin beta 10 was synthesized by a conventional solution method. Seven peptide fragments were assembled, followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole-Me2Se in trifluoroacetic acid. In preliminary experiments the synthetic tritetracontapeptide increased the entire peripheral T-cell population and a helper T-cell subset when incubated in vitro with blood which was obtained from a uremic patient with pneumonia, but a suppressor/cytotoxic T-cell subset was unaffected under these conditions. The synthetic endo-Arg38a-deacetylthymosin beta 10 was as active as synthetic deacetylthymosin beta 10 in this in vitro assay.