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Reduced restriction of EcoK in the presence of the plasmid pKM ard+. II. Cloning of the gene ard

Iusifov, T.N.; Zavil'gel'skiĭ, G.B.; Del'ver, E.P.; Belogurov, A.A.

Molekuliarnaia Biologiia 21(3): 847-852

1987

ISSN/ISBN: 0026-8984
PMID: 2821380
Document Number: 290945
Plasmid pKM101 affects the I type restriction EcoK in E. coli. The gene ard responsible for alleviation of EcoK restriction was shown to be located within the BglIIB fragment of pKM101. Here we have cloned ard gene into high copy vectors pBR322 and pUC12. Plasmid pD12 was constructed by introducing into pUC12 a 1.87 kb HindIII-Pst fragment, carrying ard gene. Tn5 and Tn1000 insertions were obtained in the ard gene region.

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