PCSE4 plasmid for cloning of promoter-containing DNA fragments in francisella tularensis
Pomerantsev, A.P.; Pavlov, V.M.
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk 12: 29-32
1999
ISSN/ISBN: 0869-6047 PMID: 10709464 Document Number: 8071
Recombinant pCSE4 plasmid has been constructed, which contains the cat gene of the Tn9 without its own promoter and with the restriction BamH1 site in front of the Shine-Delgarno region of this gene. pCSE4 is consistently inherited in the cells of F. tularensis and E. coli and makes the cells of both microorganisms to be resistant to tetracycline. By cloning the Sau3A fragments of F. tularensis chromosomal DNA at the BamH1 site of pCSE4. Promoter-containing DNA fragments were selected. The plasmids with such chromosomal DNA fragments retained their structural integrity and functional activity in F. tularensis.
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