Peptide mapping of the major proteins in 2 strains of influenza virus
Savich, I.M.; Gerasimova, L.M.; Mertvetsov, N.P.
Voprosy Virusologii 30(1): 27-32
1985
ISSN/ISBN: 0507-4088 PMID: 3922120 Document Number: 257810
The protein composition of two influenza A virus strains from different serological groups was studied. For preliminary separation of envelope and core proteins the virus was treated with nonionic detergent Triton X-100 solution followed by chromatography in ultragel AcA 34. After their separation by disk electrophoresis and treatment with trypsin they were labeled with tritium and peptide analysis was carried out. Differences in peptide maps between the appropriate light and heavy chains of hemagglutinins of the strains under study were demonstrated. Differences in the composition of nucleoprotein and matrix protein were insignificant.