On the origin of peritoneal resident macrophages. I. DNA synthesis in mouse peritoneal resident macrophages
De Bakker, J.M.; De Wit, A.W.; Onderwater, J.J.; Ginsel, L.A.; Daems, W.T.
Journal of Submicroscopic Cytology 17(2): 133-139
1985
ISSN/ISBN: 0022-4782 PMID: 3999178 Document Number: 249750
Mouse resident macrophages were isolated from the unstimulated peritoneal cavity at various intervals after one i.v. injection of [3H]-thymidine. EM autoradiography showed that 15 min after the injection, peritoneal resident macrophages, with the characteristic localization of the peroxidatic activity in the rough endoplasmic reticulum and the nuclear envelope, had already incorporated [3H]-thymidine. In the peripheral blood, monocytes with Ag grains over the nuclei were seen from 12 h onwards with a maximum at 48 h. In the peritoneal cavity labeled monocytes appeared at 24 h. The peak for labeled blood monocytes was not followed by a peak for labeled peritoneal resident macrophages. Resident macrophages from the unstimulated peritoneal cavity are able to proliferate locally.