Estrogens induce apoptosis in mouse peritoneal macrophages
Zhang, X.W.; Niu, X.L.; Guo, Z.G.
Zhongguo Yao Li Xue Bao 18(3): 267-270
1997
ISSN/ISBN: 0253-9756 PMID: 10072948 Document Number: 477847
AIM: To study whether estrogen might induce apoptosis m mouse peritoneal macrophages (MPM). METHOD: The MPM were isolated and incubated in culture medium containing 17-beta-estradiol, estrone, or equal volume of 100% ethanol as control. DNA fragmentation was visualized by agarose gel electrophoresis. RESULTS: 17-beta-Estradiol 0.01 - 1 mu-mol cntdot L-1 or estrone 10 - 20 mu-mol cntdot L-1 elicited typical morphological apoptosis and DNA fragmentation in a concentration-dependent manner in MPM. Staurosporine (Sta) 0.01 mu-mol cntdot L-1 cycloheximide (Cyc) 1 mg cntdot L-1, and tamoxifen (Tam) 10 mu-mol cntdot L-1 inhibited the DNA fragmentation induced by 17-beta-estradiol 1 mu-mol cntdot L-1 or estrone 20 mu-mol L-1. CONCLUSION: Estradiol and estrone induced apoptosis in MPM.