Effects of matrine on mouse splenocyte proliferation and release of interleukin-1 and -6 from peritoneal macrophages in vitro
Hu, Z.L.; Zhang, J.P.; Qian, D.H.; Lin, W.; Xie, W.F.; Zhang, X.R.; Chen, W.Z.
Zhongguo Yao Li Xue Bao 17(3): 259-261
1996
ISSN/ISBN: 0253-9756 PMID: 9812752 Document Number: 464815
AIM: To investigate the mechanisms of antiinflammatory effect of matrine (Mat), its effects on mouse splenocyte proliferation, and release of interleukin-1 (IL-1) and interleukin-6 (IL-6) from mouse peritoneal macrophages. METHODS: Splenocyte proliferation was assayed by (3H)TdR incorporation. IL-1 and IL-6 activities were measured by thymocyte proliferation assay and Bg cell proliferation MTT colorimetric method, respectively. RESULTS: Mat (125-500 mg cntdot L-1) obviously inhibited concanavalin A (Con A, 5 mg cntdot L-1)- and lipopolysaccarides (LPS, 10 mg cntdot L-1)-induced splenocyte proliferation and LPS-induced release of IL-1 and IL-6 from mouse peritoneal macrophages. CONCLUSION: Mat inhibited splenocyte proliferation and release of IL-1 and IL-6 in vitro.