Tumoricidal properties of rat peritoneal macrophages activated with various activators depend on nitrogen oxid synthesis
Kusenda, J.; Kalafut, F.; Klobusická, M.; Novotná, L.
Neoplasma 39(1): 15-21
1992
ISSN/ISBN: 0028-2685 PMID: 1528300 Document Number: 398432
Cytolytic activity of mineral oil elicited rat peritoneal macrophages activated by lipopolysaccharide (LPS) and/or rIFN-.gamma., rIL-2, Zymosan and PMA (4-beta-phorbol-12-beta-myristate 13-.alpha.-acetate) was detected in the presence of various concentrations of L-arginine. This paralleled the NO2- production in the presence, but not in the absence, of L-arginine. Significant amount of NO2- was detected in the peritoneal macrophages cultured with 0.4 mmol of L-arginine 8 days and the last 24 h with LPS at a concentration of 1 .mu.g/ml. No significant differences were found between activated peritoneal macrophages obtained from normal (healthy) and/or from tumor bearing rats in induce tumoricidal activity and NO2- production under the same experimental conditions. The results showed that the major cytolytic mechanism against BP6-Tu2 and U 937 tumor cell lines is L-arginine-dependent nitrogen oxide synthesis of activated rat peritoneal macrophages.