Enzyme immunoassay for urogenital trichomoniasis as a marker of unsafe sexual behaviour
Mason, P.R.; Gregson, S.; Gwanzura, L.; Cappuccinelli, P.; Rapelli, P.; Fiori, P.L.
Epidemiology and Infection 126(1): 103-109
2001
ISSN/ISBN: 0950-2688 PMID: 11293668 DOI: 10.2307/3864772Document Number: 245226
Enzyme immunoassay (EIA) was used to detect antibodies to Trichomonas vaginalis in sera from Zimbabwe . The EIA showed a sensitivity of 95 and 94% when compared with vaginal swab culture among women attending a family planning clinic (FPC; n=150) and female commercial sex workers (CSW; n=61), respectively. The specificity was 85 and 77% in the 2 groups. Culture-negative FPC women were sub-divided into high risk or low risk of exposure to trichomoniasis. The seroprevalence was 10% (6/61) among low risk women, 21% (10/48) among high risk women and 23% (9/39) among culture negative CSW. The EIA was positive in 46% (18/39) men with genital discharge but only 5% (2/37) healthy blood donors. None of 31 sera from prepubescent children was positive. The EIA may be useful for community surveys of trichomoniasis. Because T. vaginalis is a common sexually transmitted disease, the test may indicate behaviour that increases the risk of STD transmission.