Extraction of Cysts and Eggs of Heterodera schachtii from Soil with an Assessment of Extraction Efficiency
Caswell, E.P.; Thomason, I.J.; McKinney, H.E.
Journal of Nematology 17(3): 337-340
1985
ISSN/ISBN: 0022-300X PMID: 19294103 Document Number: 245227
Techniques are described for the extraction and enumeration of cysts and contained eggs from soil samples. The average recovery of cysts from seeded soil samples of differing soil texture was 82.7 +or- 2.1%. Recovery from sandy clay soil samples seeded at 1 cyst/100 g soil was 63.4 +or- 5.5%; at 4.2 cysts/100 g soil 89.6 +or- 1.8%. Recovery of cysts from naturally infested clay soil was 88.3 +or- 2.05%. Egg extraction efficiency for seeded samples was calculated as 78%, and for naturally infested soil was estimated as 83%.