Enzyme immunoassay for rapid detection of Chlamydia trachomatis in urogenital infections
Jalgaonkar, S.V.; Pathak, A.A.; Thakar, Y.S.; Kher, M.M.
Indian Journal of Sexually Transmitted Diseases 11(1): 23-26
1990
ISSN/ISBN: 0253-7184 PMID: 12284868 Document Number: 376779
An enzyme immunoassay for chlamydial antigen was tried in a pilot test of 40 patients with nongonococcal urethritis, 26 with pelvic inflammatory disease and 20 healthy controls. The EIA was a kit from Pharmacia Diagnostics. Endocervical or endourethral swabs with sticks supplied with the kit were inserted in transport tubes and kept in diluent for 10-15 minutes, the tubes were boiled 10-15 minutes, cooled and stored frozen until tested. Aliquots of the specimens and controls were incubated in kit test wells at 37 degrees C. for 30 minutes, enzyme conjugate was added for a further incubation of 45 minutes, wells were washed, and incubated with substrate for 60 minutes. Results were read on a spectrophotometer. 32.5% of the male cases, 38.5% of the female cases and none of the controls were positive. This method appears to be less costly and time consuming than culture of chlamydia, and therefore useful for screening.